The Tumor Tissue and Pathology Shared Resource (TTPSR) at Wake Forest Baptist Comprehensive Cancer Center (WFBCCC) consists of four well-integrated laboratories, all equipped with state-of-the-art instrumentation and overseen by certified pathologists and trained staff:
- Tumor Procurement Lab - Providing access to more than 44,000 vials of human tumor tissue specimens with matched adjacent normal tissues from both primary and metastatic lesions available for cancer research.
- Human Pathology Lab - Providing support for routine histological, histochemical, and immunohistochemical applications for tissues specifically collected for cancer research.
- Comparative Pathology Lab - A fully functional laboratory that emphasizes the diagnostic and experimental pathology of both rodent and nonhuman primate models of cancer.
- Organoid Processing Lab - Integrating tissue acquisition to create tumor organoids to support precision medicine research, grow and study rare cancers, support immunotherapy screening studies, and provide a personalized platform to test new drugs.
The mission of the TTPSR is to facilitate translational cancer research by:
- Collecting and supplying high-quality, well-characterized human and animal tissues (neoplastic and normal)
- Providing various pathology services to WFBCCC investigators
The TTPSR carries out this mission by:
- Maintaining a repository of patient-derived tumors and matched normal tissues in addition to tissues from other species that are accompanied by pertinent clinical information
- Facilitating investigators’ access to available tumor and normal tissues, serum, plasma, and white blood cell data via the institutional Data Warehouse and i2b2, as well as the Shared Resource’s web-based biospecimen management tool, in a regulatory-compliant manner.
- Providing scientific and pathology expertise to support the development of clinical protocols, grant applications, and manuscripts
The TTPSR also assists investigators with the customized collection of fresh human and animal tissue samples for prospective translational research projects.
Examples of Science Supported
Su W, Hong L, Xu X, Huang S, Herpai D, Li L, Xu Y, Truong L, Hu WY, Wu X, Xiao C, Xhang W, Han J, Debinski W, Xiang R, Sun P. (2018). “miR-30 disrupts senescence and promotes cancer by targeting both p16(INK4A) and DNA damage pathways.” Oncogene: 37(42): 5618-5632.
Rimkus TK, Carpenter RL, Sirkisoon S, Zhu D, Pasche BC, Chan MD, Lesser GJ, Tatter SB, Watabe K, Debinski W, Lo HW . (2018). “Truncated Glioma-Associated Oncogene Homolog 1 (tGLI1) Mediates Mesenchymal Glioblastoma via Transcriptional Activation of CD44.” Cancer Res; 78(10):2589-600.
Andrews, RN, Caudell DL, Metheny-Barlow LJ, Peiffer AM, Tooze JA, Bourland JD, Hampson RE, Deadwyler SA, Cline JM. (2018). "Fibronectin Produced by Cerebral Endothelial and Vascular Smooth Muscle Cells Contributes to Perivascular Extracellular Matrix in Late-Delayed Radiation-Induced Brain Injury." Radiat Res 190(4): 361-373.
Farris M, McTyre ER, Okoukoni C, Dugan G, Johnson BJ, Blackstock AW, Munley MT, Bourland JD, Cline JM, Willey JS. (2018). "Cortical Thinning and Structural Bone Changes in Non-Human Primates after Single-Fraction Whole-Chest Irradiation." Radiat Res 190(1): 63-71.
Mao C, Li F, Zhao Y, Debinski W, Ming X. (2018). "P-glycoprotein-targeted photodynamic therapy boosts cancer nanomedicine by priming tumor microenvironment." Theranostics 8(22): 6274-6290.
Rimkus TK, Carpenter RL, Sirkisoon S, Zhu D, Pasche BC, Chan MD, Lesser GJ, Tatter SB, Watabe K, Debinski W, Lo HW. (2018). "Truncated Glioma-Associated Oncogene Homolog 1 (tGLI1) Mediates Mesenchymal Glioblastoma via Transcriptional Activation of CD44." Cancer Res 78(10): 2589-2600.
Sirkisoon SR, Carpenter RL, Rimkus T, Anderson A, Harrison A, Lange AM, Jin G, Watabe K, Lo HW. (2018). "Interaction between STAT3 and GLI1/tGLI1 oncogenic transcription factors promotes the aggressiveness of triple-negative breast cancers and HER2-enriched breast cancer." Oncogene 37(19): 2502-2514.
Su W, Hong L, Xu X, Huang S, Herpai D, Li L, Xu Y, Truong L, Hu WY, Wu X, Xiao C, Zhang W, Han J, Debinski W, Xiang R, Sun P. (2018). "miR-30 disrupts senescence and promotes cancer by targeting both p16(INK4A) and DNA damage pathways." Oncogene 37(42): 5618-5632.
Xing F, Liu Y, Wu SY, Wu K, Sharma S, Mo YY, Feng J, Sanders S, Jin G, Singh R, Vidi PA, Tyagi A, Chan MD, Ruiz J, Debinski W, Pasche BC, Lo HW, Metheny-Barlow LJ, D’Agostino RB Jr, Watabe K. (2018). "Loss of XIST in Breast Cancer Activates MSN-c-Met and Reprograms Microglia via Exosomal miRNA to Promote Brain Metastasis." Cancer Res 78(15): 4316-4330.
Cline JM, Dugan G, Bourland JD, Perry DL, Stitzel JD, Weaver AA, Jiang C, Tovmasyan A, Owzar k, Spasojevic I, Batinic-Haberle I, Vujaskovic Z . (2018). "Post-Irradiation Treatment with a Superoxide Dismutase Mimic, MnTnHex-2-PyP(5+), Mitigates Radiation Injury in the Lungs of Non-Human Primates after Whole-Thorax Exposure to Ionizing Radiation." Antioxidants (Basel) 7(3).
Atkins HM, Appt SE, Taylor RN, Torres-Mendoza Y, Lenk EE, Rosenthal NS, Caudell DL. (2018). "Systemic Iron Deficiency in a Nonhuman Primate Model of Endometriosis." Comp Med 68(4): 298-307.
Balamayooran G, Atkins HM, Whitlow CT, Aycock ST, Nader MA, Cline JM, Caudell DL. (2018). "Labyrinthitis Ossificans in a Cynomolgus Macaque (Macaca fascicularis)." Comp Med. doi: 10.30802/AALAS-CM-17-000070. [Epub ahead of print]
Votanopoulos KI, Mazzocchi A, Sivakumar H, Forsythe S, Aleman J, Levine EA, Skardal A. (2019). "Appendiceal Cancer Patient-Specific Tumor Organoid Model for Predicting Chemotherapy Efficacy Prior to Initiation of Treatment: A Feasibility Study." Ann Surg Oncol 26(1): 139-147.
Mazzocchi A, Devarasetty M, Herberg S, Petty WJ, Marini F, Miller L, Kucera G, Dukes DK, Ruiz J, Skardal A, Soker S. Pleural Effusion Aspirate for Use in 3D Lung Cancer Modeling and Chemotherapy Screening. ACS Biomater. Sci. Eng. 2019;5(4):1937-1943. DOI: 10.1021/acsbiomaterials.8b01356.
Song, Q, Hawkins, GA, Wudel, L, Chou, PC, Forbes, E, Pullikuth, AK, Liu, L, Jin, G, Craddock, L, Topaloglu, U, Kucera, G, O'Neill, S, Levine, EA, Sun, P, Watabe, K, Lu, Y, Alexander-Miller, MA, Pasche, B, Miller, LD, Zhang, W. Dissecting intratumoral myeloid cell plasticity by single cell RNA-seq. Cancer Med. 2019:8:3072-3085. PMID: 31033233.
Sexton, T, Kucera, G, Levine, EA, Watabe, K, O’Neill, SS. Optimization of Tissue Microarrays from Banked Human Formalin-Fixed Paraffin Embedded Tissues in the Cancer Research Setting. Biopreserv. Biobank 2019:Epub ahead of print. PMID: 31194582.
Claus C, Ferrara C, Xu W, Sam J, Lang S, Uhlenbrock F, Albrecht R, Herter S, Schlenker R, Hüsser T, Diggelmann S, Challier J, Mössner E, Hosse RJ, Hofer T, Brünker P, Joseph C, Benz J, Ringler P, Stahlberg H, Lauer M, Perro M, Chen S, Küttel C, Bhavani Mohan PL, Nicolini V, Birk MC, Ongaro A, Prince C, Gianotti R, Dugan G, Whitlow CT, Solingapuram Sai KK, Caudell DL, Burgos-Rodriguez AG, Cline JM, Hettich M, Ceppi M, Giusti AM, Crameri F, Driessen W, Morcos PN, Freimoser-Grundschober A, Levitsky V, Amann M, Grau-Richards S, von Hirschheydt T, Tournaviti S, Mølhøj M, Fauti T, Heinzelmann-Schwarz V, Teichgräber V, Colombetti S, Bacac M, Zippelius A, Klein C, Umaña P. Tumor-targeted 4-1BB agonists for combination with T cell bispecific antibodies as off-the-shelf therapy. Sci Transl Med. 2019:11:496. PMID: 31189721.
Jimenez H, Wang M, Zimmerman JW, Pennison MJ, Sharma S, Surratt T, Xu ZX, Brezovich I, Absher D, Myers RM, DeYoung B, Caudell DL, Chen D, Lo HW, Lin HK, Godwin DW, Olivier M, Ghanekar A, Chen K, Miller LD, Gong Y, Capstick M, D'Agostino RB Jr, Munden R, Merle P, Barbault A, Blackstock AW, Bonkovsky HL, Yang GY, Jin G, Liu L, Zhang W, Watabe K, Blackman CF, Pasche BC. Tumour-specific amplitude-modulated radiofrequency electromagnetic fields induce differentiation of hepatocellular carcinoma via targeting Ca(v)3.2 T-type voltage-gated calcium channels and Ca(2+) influx. EBioMedicine. 2019:44:209-224. PMCID: PMC6604666.