The Flow Cytometry Shared Resource provides researchers with ready access to the necessary expertise and state-of-the-art instrumentation they need to explore a wide range of research problems with flow cytometry.

We focus on addressing the needs of research scientists and clinical investigators alike, whether they are seasoned flow cytometry users or just discovering the capabilities of flow cytometry technology. The WFL-FCSR critically enables studies at the single-cell level across a broad range of cytometry applications, particularly in immuno-oncology. We provide training for the instruments for new users and laboratory support for clinical research. Our directors are also available to consult on experimental design, development of correlative research endpoints to clinical trials and data analysis.

Focus Areas

  • Versatile Flow Cytometry experiment designs from single markers testing to high multiplexing analyses. Some representative examples of analysis experiments include: cell marker expression (surface, intracellular or intranuclear), cell viability, apoptosis, cell cycle, reactive oxygen species production, intracellular ion concentration. Cell lysates can also be analyzed with bead substrate-based immunoassays.
  • Single cell or bulk sorting of fluorescently labeled cells. Some representative examples of sorting experiments include isolation of GFP-tagged cells, isolation of multiple cell subsets from a complex sample.
  • Drop off testing of clinical specimens including sample fractioning, immunoassay, flow cytometry panel development, validation, and optimization (including CAP/CLIA assay).
  • Machine learning applications to high dimensional data and rare event cytometry analyses.