The Flow Cytometry Shared Resource provides researchers with ready access to the necessary expertise and state-of-the-art instrumentation they need to explore a wide range of research problems with flow cytometry.
We focus on addressing the needs of seasoned flow cytometry users as well as those just discovering the capabilities of flow cytometry technology. We provide monthly training for the instruments for new users. Our directors are also available to consult on experimental design and data analysis.
Typical experiments handled by the Flow Cytometry Shared Resource facility are designed to analyze or to sort based on up to nine fluorescent parameters. Some representative examples of analysis experiments include the:
- Measurement of DNA content for cell cycle analysis
- Cell cycle-specific nuclear and cytoplasmic proteins
- Apoptosis markers
- Substituted deoxyuridine incorporation
- Stem cell side populations
- Reactive oxygen species
- Cell surface markers using labeled antibodies or substrates
- Cell viability
- Intracellular ion concentration or pH
- Membrane potential
Cell lysates can also be analyzed with bead substrate-based immunoassays. Some representative examples of sorting experiments include:
- Purification of cells transfected with fluorescent protein-tagged DNA inserts
- Isolation of cell populations expressing specific intracellular or membrane metabolic products
Program key: CPC = Cancer Prevention and Control Program; CRP = Clinical Research Program; TPR = Tumor Progression and Recurrence Program; CBB = Cancer Biology and Biochemistry Program
Birbrair A, Sattiraju A, Zhu D, Zulato G, Batista I, Nguyen VT, Messi ML, Solingapuram Sai KK, Marini FC, Delbono O, Mintz A. Novel peripherally derived neural-like stem cells as therapeutic carriers for treating glioblastomas. Stem Cells Transl Med. 2017 Feb;6(2):471-481. doi: 10.5966/sctm.2016-0007. Epub 2016 Sep 14. PMID: 28191774 http://onlinelibrary.wiley.com/doi/10.5966/sctm.2016-0007/epdf
Wu K, Fukuda K, Xing F, Zhang Y, Sharma S, Liu Y, Chan MD, Zhou X, Qasem SA, Pochampally R, Mo YY, Watabe K. Roles of the cyclooxygenase 2 matrix metalloproteinase 1 pathway in brain metastasis of breast cancer. J. Biol. Chem. 2015;290(15):9842-54. PMC4392281.
Swanner J, Mims J, Carroll DL, Akman SA, Furdui CM, Torti SV, Singh RN. Differential cytotoxic and radiosensitizing effects of silver nanoparticles on triple-negative breast cancer and non-triple-negative breast cells. Int J Nanomedicine. 2015;10:3937-53. PMC4501353.