Equipment |
Cost |
Assisted/Unassisted |
IVIS Lumina III |
$65/hr |
Unassisted - trained users only |
IncuCyte Zoom |
$0.63/hr |
Unassisted - trained users only |
BioSpherix Xvivo X3 Hypoxia Chamber |
$0.63/hr |
Unassisted - trained users only |
Nanosight NS500 |
Contact us |
Assisted |
Training for all instruments is available upon request |
Free |
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CELL CULTURE SERVICES |
Cost |
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Mycoplasma Testing |
Price depends on sample number |
3 samples: $28/ea |
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8 samples: $18/ea |
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13 samples: $15.55/ea |
Cancer Cell Line (frozen vial) |
$33 |
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Cancer Cell Line (reestablished in T75 flask) |
$50.00 |
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Liquid nitrogen storage (per month for 1 box) |
$9.10 |
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Custom Media Formulation |
Contact us |
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CELL CULTURE REAGENTS |
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Contact us for a list of discounted supplies including FBS, media, additives and plastics. |
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Orders placed daily. |
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PRIMER ORDERS FROM INVITROGEN |
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Contact us for custom primer order forms |
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SEQUENCING FROM GENEWHIZ |
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Contact us for more information |
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VIRAL VECTORS |
Cost |
Applies to each LV: |
Concentrated, premade lentivirus (in stock) |
50 µL vial of 100X concentrated LV |
Buy 1, $150 |
CMV-GFP; puromycin selectable |
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Buy 2, $140 each |
CMV-mCHERRY; puromycin selectable |
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Buy 3, $130 ea |
CMV-Luciferase; blastocidin selectable |
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Buy 4, $120 ea |
CMV-Luciferase; puromycin selectable |
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Buy 5, $110 ea |
CMV-Cas9-EGFP; zeocin selectable (expresses Cas9 protein and EGFP from EFS promoter) |
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Buy 6, $100 ea |
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Custom 2nd generation lentivirus production (Δ8.8/VSV-G); transfer vector provided by PI |
Small (40 mL) |
Large (100 mL) |
Lentiviral supernatant |
$270 |
$345 |
Lentiviral supernatant + GoStix Titer |
$290 |
$365 |
Lentiviral supernatant + Spinfection Titer |
$500 |
$580 |
Lentiviral supernatant + Spinfection Titer + GoStix Titer |
$520 |
$600 |
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Concentrated lentivirus |
$486 |
$593 |
Concentrated lentivirus + GoStix Titer |
$506 |
$613 |
Concentrated lentivirus + Spinfection Titer |
$720 |
$826 |
Concentrated lentivirus + Spinfection Titer + GoStix Titer |
$740 |
$846 |
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Custom 3rd generation lentivirus production (pPACKH1 mix); transfer vector provided by PI |
Small (40 mL) |
Large (100 mL) |
Lentiviral supernatant |
$400 |
$655 |
Lentiviral supernatant + GoStix Titer |
$420 |
$675 |
Lentiviral supernatant + Spinfection Titer |
$625 |
$890 |
Lentiviral supernatant + Spinfection Titer + GoStix Titer |
$645 |
$910 |
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Concentrated lentivirus |
$600 |
$900 |
Concentrated lentivirus + GoStix Titer |
$620 |
$920 |
Concentrated lentivirus + Spinfection Titer |
$840 |
$1,130 |
Concentrated lentivirus + Spinfection Titer + GoStix Titer |
$860 |
$1,150 |
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Custom Retrovirus/AAV production |
Contact us for a cost estimate ($65/h for labor plus reagents) |
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Plasmid Preparation |
(Labor included; multiple preps are batched making pricing not a straight one to one; eg. 3 x Mini-preps = $70) |
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Mini Prep |
One Mini-prep nothing else = $65 |
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Midi Prep |
One MIDI-prep nothing else = $76 |
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Maxi Prep |
One MAXI-prep nothing else = $90 |
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Endotoxicin-free Maxi Prep |
One Endo-free MAXI-prep nothing else = $150 |
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Restriction Analysis |
Add in validation to any prep = $160 |
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Transformation |
Add in transformation, includes colony to plate to 5mL LB = $70-80 (depends on E. coli strain used, eg. DH5alpha cheaper than Stbl3) |
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OTHER SERVICES |
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CRISPR design and optimization/gRNA validation |
Cost |
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Partnered with Sigma-Aldrich’s CRISPR Core Partnership Program |
Consultation is free. Contact us for a cost estimate ($65/h for labor plus reagents) |
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In-house validation of gRNAs |
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PCR-based ID of insertions/deletions generated in genome of cells |
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treated with engineered nucleases (ZFNs, TALENs, CRISPR/Cas9) |
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Amplicon melted, hybridized to form mismatched targets for cleavage |
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by the Guide-it Resolvase. Cleavage efficiency determined by analyzing |
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samples on agarose gel followed by densitometric analysis |
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Gateway Cloning |
Cost |
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TOPO cloning |
Consultation is free. Contact us for a cost estimate ($65/h for labor plus reagents) |
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Primer design for blunt-end PCR product with 3’ single-stranded |
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overhang for directional cloning into pENTR/D-TOPO vector |
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to generate Entry Clone |
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LR Clonase II reaction |
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Entry Clone (attL sites) + Gateway Destination Vector (attR sites) |
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to generate Expression Clone |
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for LV prep and/or transfection of target cells |
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