| Equipment | Cost | Assisted/Unassisted |
| IVIS Lumina III | $65/hr | Unassisted - trained users only |
| IncuCyte Zoom | $0.63/hr | Unassisted - trained users only |
| BioSpherix Xvivo X3 Hypoxia Chamber | $0.63/hr | Unassisted - trained users only |
| Nanosight NS500 | Contact us | Assisted |
| Training for all instruments is available upon request | Free | |
| CELL CULTURE SERVICES | Cost | |
| Mycoplasma Testing | Price depends on sample number | 3 samples: $28/ea |
| 8 samples: $18/ea | ||
| 13 samples: $15.55/ea | ||
| Cancer Cell Line (frozen vial) | $33 | |
| Cancer Cell Line (reestablished in T75 flask) | $50.00 | |
| Liquid nitrogen storage (per month for 1 box) | $9.10 | |
| Custom Media Formulation | Contact us | |
| CELL CULTURE REAGENTS | ||
| Contact us for a list of discounted supplies including FBS, media, additives and plastics. | ||
| Orders placed daily. | ||
| PRIMER ORDERS FROM INVITROGEN | ||
| Contact us for custom primer order forms | ||
| SEQUENCING FROM GENEWHIZ | ||
| Contact us for more information | ||
| VIRAL VECTORS | Cost | Applies to each LV: |
| Concentrated, premade lentivirus (in stock) | 50 µL vial of 100X concentrated LV | Buy 1, $150 |
| CMV-GFP; puromycin selectable | Buy 2, $140 each | |
| CMV-mCHERRY; puromycin selectable | Buy 3, $130 ea | |
| CMV-Luciferase; blastocidin selectable | Buy 4, $120 ea | |
| CMV-Luciferase; puromycin selectable | Buy 5, $110 ea | |
| CMV-Cas9-EGFP; zeocin selectable (expresses Cas9 protein and EGFP from EFS promoter) | Buy 6, $100 ea | |
| Custom 2nd generation lentivirus production (Δ8.8/VSV-G); transfer vector provided by PI | Small (40 mL) | Large (100 mL) |
| Lentiviral supernatant | $270 | $345 |
| Lentiviral supernatant + GoStix Titer | $290 | $365 |
| Lentiviral supernatant + Spinfection Titer | $500 | $580 |
| Lentiviral supernatant + Spinfection Titer + GoStix Titer | $520 | $600 |
| Concentrated lentivirus | $486 | $593 |
| Concentrated lentivirus + GoStix Titer | $506 | $613 |
| Concentrated lentivirus + Spinfection Titer | $720 | $826 |
| Concentrated lentivirus + Spinfection Titer + GoStix Titer | $740 | $846 |
| Custom 3rd generation lentivirus production (pPACKH1 mix); transfer vector provided by PI | Small (40 mL) | Large (100 mL) |
| Lentiviral supernatant | $400 | $655 |
| Lentiviral supernatant + GoStix Titer | $420 | $675 |
| Lentiviral supernatant + Spinfection Titer | $625 | $890 |
| Lentiviral supernatant + Spinfection Titer + GoStix Titer | $645 | $910 |
| Concentrated lentivirus | $600 | $900 |
| Concentrated lentivirus + GoStix Titer | $620 | $920 |
| Concentrated lentivirus + Spinfection Titer | $840 | $1,130 |
| Concentrated lentivirus + Spinfection Titer + GoStix Titer | $860 | $1,150 |
| Custom Retrovirus/AAV production | Contact us for a cost estimate ($65/h for labor plus reagents) | |
| Plasmid Preparation | (Labor included; multiple preps are batched making pricing not a straight one to one; eg. 3 x Mini-preps = $70) | |
| Mini Prep | One Mini-prep nothing else = $65 | |
| Midi Prep | One MIDI-prep nothing else = $76 | |
| Maxi Prep | One MAXI-prep nothing else = $90 | |
| Endotoxicin-free Maxi Prep | One Endo-free MAXI-prep nothing else = $150 | |
| Restriction Analysis | Add in validation to any prep = $160 | |
| Transformation | Add in transformation, includes colony to plate to 5mL LB = $70-80 (depends on E. coli strain used, eg. DH5alpha cheaper than Stbl3) | |
| OTHER SERVICES | ||
| CRISPR design and optimization/gRNA validation | Cost | |
| Partnered with Sigma-Aldrich’s CRISPR Core Partnership Program | Consultation is free. Contact us for a cost estimate ($65/h for labor plus reagents) | |
| In-house validation of gRNAs | ||
| PCR-based ID of insertions/deletions generated in genome of cells | ||
| treated with engineered nucleases (ZFNs, TALENs, CRISPR/Cas9) | ||
| Amplicon melted, hybridized to form mismatched targets for cleavage | ||
| by the Guide-it Resolvase. Cleavage efficiency determined by analyzing | ||
| samples on agarose gel followed by densitometric analysis | ||
| Gateway Cloning | Cost | |
| TOPO cloning | Consultation is free. Contact us for a cost estimate ($65/h for labor plus reagents) | |
| Primer design for blunt-end PCR product with 3’ single-stranded | ||
| overhang for directional cloning into pENTR/D-TOPO vector | ||
| to generate Entry Clone | ||
| LR Clonase II reaction | ||
| Entry Clone (attL sites) + Gateway Destination Vector (attR sites) | ||
| to generate Expression Clone | ||
| for LV prep and/or transfection of target cells | ||
