Cell Engineering Shared Resource Pricing

Equipment Cost Assisted/Unassisted
IVIS Lumina III $65/hr Unassisted - trained users only
IncuCyte Zoom $0.63/hr Unassisted - trained users only
BioSpherix Xvivo X3 Hypoxia Chamber $0.63/hr Unassisted - trained users only
Nanosight NS500 Contact us Assisted
Training for all instruments is available upon request Free
CELL CULTURE SERVICES Cost
Mycoplasma Testing Price depends on sample number 3 samples: $28/ea
8 samples: $18/ea
13 samples: $15.55/ea
Cancer Cell Line (frozen vial) $33
Cancer Cell Line (reestablished in T75 flask) $50.00
Liquid nitrogen storage (per month for 1 box) $9.10
Custom Media Formulation Contact us
CELL CULTURE REAGENTS
Contact us for a list of discounted supplies including FBS, media, additives and plastics.
Orders placed daily.
PRIMER ORDERS FROM INVITROGEN
Contact us for custom primer order forms
SEQUENCING FROM GENEWHIZ
Contact us for more information
VIRAL VECTORS Cost Applies to each LV:
Concentrated, premade lentivirus (in stock) 50 µL vial of 100X concentrated LV Buy 1, $150
CMV-GFP; puromycin selectable Buy 2, $140 each
CMV-mCHERRY; puromycin selectable Buy 3, $130 ea
CMV-Luciferase; blastocidin selectable Buy 4, $120 ea
CMV-Luciferase; puromycin selectable Buy 5, $110 ea
CMV-Cas9-EGFP; zeocin selectable (expresses Cas9 protein and EGFP from EFS promoter) Buy 6, $100 ea
Custom 2nd generation lentivirus production (Δ8.8/VSV-G); transfer vector provided by PI Small (40 mL) Large (100 mL)
Lentiviral supernatant $270 $345
Lentiviral supernatant + GoStix Titer $290 $365
Lentiviral supernatant + Spinfection Titer $500 $580
Lentiviral supernatant + Spinfection Titer + GoStix Titer $520 $600
Concentrated lentivirus $486 $593
Concentrated lentivirus + GoStix Titer $506 $613
Concentrated lentivirus + Spinfection Titer $720 $826
Concentrated lentivirus + Spinfection Titer + GoStix Titer $740 $846
Custom 3rd generation lentivirus production (pPACKH1 mix); transfer vector provided by PI Small (40 mL) Large (100 mL)
Lentiviral supernatant $400 $655
Lentiviral supernatant + GoStix Titer $420 $675
Lentiviral supernatant + Spinfection Titer $625 $890
Lentiviral supernatant + Spinfection Titer + GoStix Titer $645 $910
Concentrated lentivirus $600 $900
Concentrated lentivirus + GoStix Titer $620 $920
Concentrated lentivirus + Spinfection Titer $840 $1,130
Concentrated lentivirus + Spinfection Titer + GoStix Titer $860 $1,150
Custom Retrovirus/AAV production Contact us for a cost estimate ($65/h for labor plus reagents)
Plasmid Preparation (Labor included; multiple preps are batched making pricing not a straight one to one; eg. 3 x Mini-preps = $70)
Mini Prep One Mini-prep nothing else = $65
Midi Prep One MIDI-prep nothing else = $76
Maxi Prep One MAXI-prep nothing else = $90
Endotoxicin-free Maxi Prep One Endo-free MAXI-prep nothing else = $150
Restriction Analysis Add in validation to any prep = $160
Transformation Add in transformation, includes colony to plate to 5mL LB = $70-80 (depends on E. coli strain used, eg. DH5alpha cheaper than Stbl3)
OTHER SERVICES
CRISPR design and optimization/gRNA validation Cost
Partnered with Sigma-Aldrich’s CRISPR Core Partnership Program Consultation is free. Contact us for a cost estimate ($65/h for labor plus reagents)
In-house validation of gRNAs
PCR-based ID of insertions/deletions generated in genome of cells
treated with engineered nucleases (ZFNs, TALENs, CRISPR/Cas9)
Amplicon melted, hybridized to form mismatched targets for cleavage
by the Guide-it Resolvase. Cleavage efficiency determined by analyzing
samples on agarose gel followed by densitometric analysis
Gateway Cloning Cost
TOPO cloning Consultation is free. Contact us for a cost estimate ($65/h for labor plus reagents)
Primer design for blunt-end PCR product with 3’ single-stranded
overhang for directional cloning into pENTR/D-TOPO vector
to generate Entry Clone
LR Clonase II reaction
Entry Clone (attL sites) + Gateway Destination Vector (attR sites)
to generate Expression Clone
for LV prep and/or transfection of target cells