Chromatin Motions and Genomic Translocations

Chromatin is in constant motion in the cell nucleus. The mechanisms regulating these motions and their functional consequences are largely unknown.

This project addresses how chromatin motions are regulated in response to DNA damage and tests the hypothesis that chromatin motions are necessary for (and could predict) the biogenesis of genomic translocations. 

7 x 7 chromatin PA

We developed a method based on structured illumination to track the motions of chromatin microdomains and found that chromatin diffusion fluctuates after DNA damage (Liu et al., J Cell Sci, 2015; Bonin et al., JBO, 2018).

Our goal is to better understand the cellular mechanisms regulating chromatin motions and the functional significance of chromatin motions for genome functions. This knowledge may help prevent secondary cancers (typically leukemia with poor prognoses) driven by translocation events in patients treated for solid tumors with DNA-damaging radio- and chemotherapy.

Funding

National Cancer Institute, Physical Sciences in Oncology U01 grant (2018-2023)

Selected Publications

Structured illumination to spatially map chromatin motions. Bonin K, Smelser A, Moreno NS, Holzwarth G, Wang K, Levy P, Vidi PA. J Biomed Opt. 2018 May; 23(5):1-8.

Nanoscale histone localization in live cells reveals reduced chromatin mobility in response to DNA damage. Liu J, Vidi PA, Lelièvre SA, Irudayaraj JM. J Cell Sci. 2015 Feb 01; 128(3):599-604.