The Bishop laboratory utilizes metabolomics approaches to identify and track metabolic changes as a result of disease initiation, progression and treatment. This work focuses on both biased and unbiased metabolite analysis of bio-volatiles and biofluids in biological systems ranging from cell-based experiments to patient samples.

Ongoing research projects:

  1. Utilizing exhaled breath and biofluid metabolites for early detection of disease (e.g. lung cancer and diabetes)
  2. Assessing exhaled breath and biofluid metabolites to monitor disease treatments
  3. Understanding biochemical origins of bio-volatiles from exhaled breath

Research Highlights

Metabolomics Analysis of a Breath and Biofluid

Metabolomics has emerged as an important contributor to a systems biology approach of studying complex diseases. Metabolism is a key function of every cellular process, and tools to globally capture a comprehensive fingerprint of metabolism play a major role in understanding the important mechanism involved. Therefore, it is critical to establish standardized methods for metabolomics analysis that can be utilized to gain information from any biological material.

I am currently using a metabolomics approach to explore breath and biofluid metabolites as a way to discover potential signatures of cardiac dysfunction and cancer. In a model of developmental programming, these metabolic dysregulations are accelerated, allowing us to use this platform to study a disease in a highly controllable setting. In a collaborative project with Children’s Hospital of San Antonio and Baylor University, we are assessing breath and serum metabolites from prediabetic children from the San Antonio area. This study utilizes the state-of-the-art ReCIVA breath collection device from Owlstone Biomedical. By assessing the metabolic state prior to weight management treatment, we will gain metabolic information of prediabetic state of this cohort of children. This establishment of metabolomics analysis will be applicable to studying other complex diseases.

Bishop AC, Libardoni M, Misra B, Choudary A, Lange K, Bernal J, Nijland M, Olivier M, Li C, Nathanielsz  PW, Cox LA. Nonhuman primate breath metabolites correlate with developmental programming and cardio-metabolic status. J Breath Res. 2018;12 036016 doi: 10.1088/1752-7163/aaba84.

Misra BB, Bassey E, Bishop AC, Kusel DT, Cox LA, Olivier M. High-Resolution gas chromatography/mass spectrometry metabolomics of non-human primate serum. Rapid Commun Mass Spectrom. 2018; 1-10: doi: 10.1002/rcm.8197.

Areas of Research Focus

Metabolomics, Gas Chromatography Mass Spectrometry, Liquid Chromatography Mass Spectrometry, Exhaled Breath Collection, Data Analysis