The Proteomics and Metabolomics Shared Resource provides broad mass spectrometry services to academic and industry investigators.

The shared resource is affiliated with the Wake Forest Baptist Medical Comprehensive Cancer Center (WFBMCCC). Our mission is to provide access to advanced mass spectrometry techniques for quantitation and identification of proteins and metabolites in biological samples.

Services Provided

Our services range from extraction of biomolecules from complex biological or clinical specimens to analysis by mass spectrometry and primary data analysis. Specialized methods for isolation, characterization, and multi-omics analysis of exosomes, including selective analysis of surface exposed proteins, are available. We also provide options for development of new methods (e.g., drugs for pharmacokinetics studies and selected classes of metabolites, lipids or specific proteins of interest) to meet the need for specific projects.

Highlighted Equipment

  • Thermo Q Exactive™ HF Hybrid Quadrupole-Orbitrap Mass Spectrometer w/ Vanquish UHPLC and Dionex Ultimate3000 nanoLC
  • Shimazdu LCMS-8050 triple quadrupole mass spectrometer w/ Shimazdu UFLC system
  • Thermo Scientific TSQ Quantum XLS GC/MS with Trace Ultra gas chromatograph, and Triplus auto-sampler
  • Thermo Scientific TSQ Quantum Discovery Max with HP/Agilent 1100 HPLC and HP/Agilent auto-sampler
  • Analytical HPLC of Biomolecules: Columns: 2.1mm, 3.9mm, 4.6mm; C4, C18; Size Exclusion (TSK) 2000,3000,4000. Automated temperature control column heater
  • Waters 650E HPLC and LC150 System for protein purification
  • Bruker Auto-flex MALDI-TOF