The Proteomics and Metabolomics Shared Resource provides broad mass spectrometry services to academic and industry investigators.
The shared resource is affiliated with the Wake Forest Baptist Medical Comprehensive Cancer Center (WFBMCCC). Our mission is to provide access to advanced mass spectrometry techniques for quantitation and identification of proteins and metabolites in biological samples.
Our services range from extraction of biomolecules from complex biological or clinical specimens to analysis by mass spectrometry and primary data analysis. Specialized methods for isolation, characterization, and multi-omics analysis of exosomes, including selective analysis of surface exposed proteins, are available. We also provide options for development of new methods (e.g., drugs for pharmacokinetics studies and selected classes of metabolites, lipids or specific proteins of interest) to meet the need for specific projects.
- Thermo Q Exactive™ HF Hybrid Quadrupole-Orbitrap Mass Spectrometer w/ Vanquish UHPLC and Dionex Ultimate3000 nanoLC
- Shimazdu LCMS-8050 triple quadrupole mass spectrometer w/ Shimazdu UFLC system
- Thermo Scientific TSQ Quantum XLS GC/MS with Trace Ultra gas chromatograph, and Triplus auto-sampler
- Thermo Scientific TSQ Quantum Discovery Max with HP/Agilent 1100 HPLC and HP/Agilent auto-sampler
- Analytical HPLC of Biomolecules: Columns: 2.1mm, 3.9mm, 4.6mm; C4, C18; Size Exclusion (TSK) 2000,3000,4000. Automated temperature control column heater
- Waters 650E HPLC and LC150 System for protein purification
- Bruker Auto-flex MALDI-TOF
- Automated analytical HPLC analysis on a variety of chromatography columns with UV/VIS, PDA, Fluorescence, and Wyatt: SEC-MALS and QELS detection
- Manual HPLC from semi-micro to preparative
- HPLC gel filtration analysis
- Untargeted and targeted metabolomics
- Lipidomics and targeted analysis of selected classes or individual lipids (e.g., total and free cholesterol, oxysterols, cholesteryl esters
- Total and free fatty acids
- HETE/Prostaglandin metabolites)
- Drug monitoring for pharmacokinetics studies
- Quantitative analysis of nutrients or other small molecules.
Accurate measurements of protein molecular weight, protein identification from immunoprecipitation studies (interactome), absolute or relative quantification of targeted proteins of interest (e.g., AQUA methods, angiotensin species in blood), large-scale relative quantification of proteomes (label free, TMT, SILAC, super-SILAC), and qualitative or quantitative analysis of post-translational modifications (e.g., oxidation, phosphorylation, acetylation, ubiquitination).
These studies are performed on a variety of biological and clinical specimens including cells, tissues, biological fluids (urine, blood/plasma, cerebrospinal fluid), plant extracts, and other matrices. Specific methods for sample processing such as subcellular fractionation or exosomes isolation are available and interfaced with metabolomics or proteomics analysis (e.g., analysis of surface exposed exosomal proteins). Method development options that use mass spectrometry are available to fit the specifics of individual projects."
Hill TK, Abdulahad A, Kelkar SS, Marini FC(TPR), Long TE, Provenzale JM, Mohs AM(CBB). Indocyanine Green-Loaded Nanoparticles for Image-Guided Tumor Surgery. Bioconjug Chem. 2015;26(2): 294-303. PMC4373659.
Mims J, Bansal N, Bharadwaj MS, Chen X, Molina AJ, Tsang AW(TPR), Furdui CM(CBB). Energy Metabolism in a Matched Model of Radiation Resistance for Head and Neck Squamous Cell Cancer. Radiat Res. 2015;183(3): 291-304. PMC4465128