The Flow Cytometry Shared Resource provides researchers with ready access to the necessary expertise and state-of-the-art instrumentation they need to explore a wide range of research problems with flow cytometry.

We focus on addressing the needs of seasoned flow cytometry users as well as those just discovering the capabilities of flow cytometry technology. We provide monthly training for the instruments for new users. Our directors are also available to consult on experimental design and data analysis.

Focus Areas

Typical experiments handled by the Flow Cytometry Shared Resource facility are designed to analyze or to sort based on up to nine fluorescent parameters. Some representative examples of analysis experiments include the:

  • Measurement of DNA content for cell cycle analysis
  • Cell cycle-specific nuclear and cytoplasmic proteins
  • Apoptosis markers
  • Substituted deoxyuridine incorporation
  • Stem cell side populations
  • Reactive oxygen species
  • Cell surface markers using labeled antibodies or substrates
  • Cell viability
  • Intracellular ion concentration or pH
  • Membrane potential

Cell lysates can also be analyzed with bead substrate-based immunoassays. Some representative examples of sorting experiments include:

  • Purification of cells transfected with fluorescent protein-tagged DNA inserts
  • Isolation of cell populations expressing specific intracellular or membrane metabolic products

Services Provided

Flow cytometry is a powerful, versatile technology that allows researchers to simultaneously analyze individual cells in a multiparameter fashion. We offer sterile cell sorting, which allows researchers to identify distinct subpopulations in a mixed population of cells and isolate up to four populations concurrently. We also provide analysis instruments, training for use of the instruments, access to analysis software, assistance in assay design and data analytics.

Use of the Instruments

Institutional instruments available include: