The Cellular Imaging Shared Resource (CISR) is a full-service microscopy laboratory providing access to equipment and technical support for electron, brightfield/fluorescence and confocal/multiphoton microscopy. It is available for both clinical and research purposes.

This resource exists in a biomedical research environment and supports numerous institutionally based investigators whose interests encompass the breadth of both basic and clinical science. The Wake Forest Baptist Comprehensive Cancer Center (WFBCCC) provides a 50% discount for microscopy services to investigators who are members.

Although the orientation is biomedical, the facility supports a wide range of research. Faculty and staff also have extensive experience in non-biomedical zoology, botany and materials science imaging.

The CISR offers:

  • User training
  • Consultation before and after projects
  • Collaboration
  • Numerous specialized research techniques

The mission of the CISR is to provide access to:

  • Advanced microscopy instruments
  • Technical support for specimen preparation
  • Scientific guidance in planning and applying advanced microscopy techniques

Services Provided

Over the past few years, new microscopy techniques have provided a more sophisticated view into the workings of complex tissues and cells. These technologies require a foundation in the use of microscopes and an understanding of the images they generate. Our team is committed to the consultation and guidance of investigators, helping them understand and apply advanced microscopy techniques to get the most meaningful results.

We provide a broad array of advanced microscopy instruments, including:

  • Digital upright and inverted fluorescence microscopes
  • Bright field, differential interference contrast (DIC), phase contrast, polarizing, fluorescence, digital multispectral microscopy
  • Laser scanning confocal microscope (LSCM)
  • Multiphoton microscopy
  • Live-cell/time-lapse microscopy
  • Laser capture microdissection
  • Single cell mincroinjection
  • Transmission electron microscopy (TEM)
  • Scanning electron microscopy (SEM)

Additional Capabilities

  • Immunolabeling for light microscopy, electron microscopy, SEM and LSCM
  • Enzyme cytochemistry
  • Cyromicrotomy and EDX compositional microanalysis
  • Image processing and analysis

Instrumentation

  • Olympus FV1200 spectral confocal system
  • Zeiss LSM510 laser scanning confocal system
  • Olympus FV1000 5 laser confocal (WFIRM)
  • Olympus FV1000MP multiphoton/intravital microscope (WFIRM)
  • computer workstations with image processing, analysis and 3-D reconstruction software (WFIRM)
  • Arcturus PixCell II laser microdissection system
  • Olympus IX-70 fluorescence microscopes
  • Olympus X63-Multispectral -CRi NUANCE EX (WFIRM) microscope
  • Zeiss Axioplan 2
  • Zeiss Axiovert time-lapse video microscopy system for cultured cells
  • Reichert Ultracut microtomes and cryoultramicrotomes
  • FEI Technai BioTwin 120 keV TEM with digital imaging
  • complete specimen preparation facilities

The Cellular Imaging Shared Resource is equipped to carry out all phases of sample preparation for electron microscopy with a staff that includes a technical engineer/manager and two technologists.