The mission of the Cell Engineering Shared Resource is to serve the scientific needs of Wake Forest Baptist Comprehensive Cancer Center (WFBCCC) researchers. The Cell Engineering Shared Resource. serves an essential function by providing investigators with considerable expertise in cell culture/cell line engineering/viral vector propagation, and the tools to analyze cell growth/death in vitro and in vivo under a wide variety of experimental conditions.
The Cell Engineering Shared Resource is capable of designing, implementing and optimizing new cell culture/transduction procedures on behalf of investigators, and can conduct short-term experiments efficiently in a cost-effective manner. The Cell Engineering Shared Resource supports the research community at all the Wake Forest Baptist Medical Center campuses, allowing investigators to make the most of their time and research dollars.
The CE offers:
- Customized assistance for cell culture/cell line engineering techniques including gene editing (CRISPR) and viral/plasmid vector transduction
- CRISPR design and optimization (in partnership with Sigma-Aldrich’s CRISPR Core Partnership Program)
- Consultation and customized assistance to aid in design, implementation and optimization and trouble-shooting cell of new cell culture procedures
- Production and concentration of replication-incompetent viruses including lentivirus (ecotrophic and amphotrophic), AAV and adenovirus
- Plasmid expansion (transformation, mini/midi/maxiprep, DNA purification)
- Quality control for each research project
- A cell line repository (over 100 cancer cell lines)
- Cryopreservation and storage of tissue culture cells (liquid nitrogen)
- Mycoplasma testing and control
- Custom media and reagent preparation under strict quality control
- An on-site stockroom with deeply discounted pricing for cell culture media (including Fetal Bovine Serum), reagents and plastics (Fisher, ISCBioexpress, Sarstedt)
- Procurement and distribution services for highly discounted products and services from external vendors including BioRad, eBioscience, Gemini, Lonza, LifeTech, PerkinElmer, Qiagen, Roche, Sigma; daily ordering for all vendors; free shipping/packaging/handling; distribution to all campuses daily
The CE distribution center provides cell culture products. Both basic and specialty media are available to investigators at a substantial cost-savings compared to the list price or the general university subsidy.
Molecular biology reagents are available from five different vendors and reflect the breadth of products sold by these vendors. Wake Forest Baptist Comprehensive Cancer Center members receive substantial subsidies on these products, and all users benefit from free shipping.
IVIS Lumina Series III
- Non-invasive (fluorescence/luminescence) imaging of cells in rodents.
- User Manual (pdf)
- Real-time, quantitative live-cell analysis
- User Manual (pdf)
Biospherix Xvivo X3 Hypoxia hood and cell culture combo
- Growth of cells in low-oxygen (hypoxic) conditions
- User Manual (pdf)
Program key: CPC = Cancer Prevention and Control Program; CRP = Clinical Research Program; TPR = Tumor Progression and Recurrence Program; CBB = Cancer Biology and Biochemistry Program 7457-66.
Xing F, Sharma S, Liu Y, Mo YY, Wu K, Zhang YY, Pochampally R, Martinez LA, Lo HW, Watabe K. miR-509 suppresses brain metastasis of breast cancer cells by modulating RhoC and TNF-alpha. Oncogene. 2015;34:4890-900. PMC4530094.
Maglic D, Stovall DB, Cline M, Fry EA, Mallakin A, Taneja P, Caudell DL, Willingham MC, Sui G, Inoue K. DMP1beta, a splice isoform of the tumour suppressor DMP1 locus, induces proliferation and progression of breast cancer. J Pathol. 2015;236(1):90-102. PMC4398631.
Vallabhaneni KC, Penfornis P, Dhule S, Guillonneau F, Adams KV, Mo YY, Xu R, Liu Y, Watabe K, Vemuri MC, Pochampally R. Extracellular vesicles from bone marrow mesenchymal stem/stromal cells transport tumor regulatory microRNA, proteins, and metabolites. Oncotarget. 2015;6(7):4953-67. PMC4467126.
Birbrair A, Zhang T, Wang ZM, Messi ML, Mintz A, Delbono O. Pericytes at the intersection between tissue regeneration and pathology. Clin Sci (Lond). 2015;128(2):81-93. PMC4200531.
Wu K, Fukuda K, Xing F, Zhang Y, Sharma S, Liu Y, Chan MD, Zhou X, Qasem SA, Pochampally R, Watabe K, et al. Roles of the cyclooxygenase 2 matrix metalloproteinase 1 pathway in brain metastasis of breast cancer. J Biol Chem. 2015;290(15):9842-54. PMC4392281.
Liu M, Allegood J, Zhu X, Seo J, Gebre AK, Boudyguina E, Cheng D, Chuang CC, Shelness GS, Spiegel S, Parks JS. Uncleaved apoM signal peptide is required for formation of large apoM/S1P-enriched HDL particles. J Biol Chem. 2015;290(12):7861-70. PMC4367285.
Hill TK, Abdulahad A, Kelkar SS, Marini FC, Long TE, Provenzale JM, Mohs AM. Indocyanine green-loaded nanoparticles for image-guided tumor surgery. Bioconjug Chem. 2015;26(2):294-303. PMC4373659.
Westcott MM, Liu J, Rajani K, D'Agostino R, Jr., Lyles DS, Porosnicu M. IFNbeta and IFNalpha2 differentially protect head and neck cancer cells from VSV-induced oncolysis. J Virol. 2015;89:7944-54. PMC4505650.
Yu N, Puckett S, Antinozzi PA, Cramer SD, Lyles DS. Changes in susceptibility to oncolytic vesicular stomatitis virus during progression of prostate cancer. J Virol. 2015;89(10):5250-63. PMC4442527.
Turner RL, Groitl P, Dobner T, Ornelles DA. Adenovirus replaces mitotic checkpoint controls. J Virol. 2015;89(9):5083-96. PMC4403466.
Swanner J, Mims J, Carroll DL, Akman SA, Furdui CM, Torti SV, Singh RN. Differential cytotoxic and radiosensitizing effects of silver nanoparticles on triple-negative breast cancer and non-triple-negative breast cells. Int J Nanomedicine. 2015;10:3937-53. PMC4501353.
Choudhary M, Naczki C, Chen W, Barlow KD, Case LD, Metheny-Barlow LJ. Tumor-induced loss of mural Connexin 43 gap junction activity promotes endothelial proliferation. BMC Cancer. 2015;15(1):427. PMC4464240.
Ornelles DA, Gooding LR, Garnett-Benson C. Neonatal infection with species C adenoviruses confirmed in viable cord blood lymphocytes. PLoS One. 2015;10(3):e0119256. PMC4357425.
Gmeiner WH, Jennings-Gee J, Stuart CH, Pardee TS. Thymineless death in F10-treated AML cells occurs via lipid raft depletion and Fas/FasL co-localization in the plasma membrane with activation of the extrinsic apoptotic pathway. Leuk Res. 2015;39(2):229-35. PMC4306618.