The goal of the Analytical Imaging Facility is to provide equipment for the acquisition and analysis of images from radioactive, fluorescent, or chemiluminescent samples such as agarose and polyacrylamide gels, membranes, microplates, and microarrays.
Use of instrumentation is available to all investigators at Wake Forest Baptist Medical Center.
Services We Provide:
The instrumentation available includes a Typhoon Trio and an Amersham Imager-600 RGB multi-label imager.
- Typhoon Trio
The Typhoon Imager allows for indirect phosphor imaging of 3H, 14C, 125I, 32P, 33P, 35S or other radioactive signals from gels or membranes. Users provide their own phosphor storage screens for these applications; Image Erasers are available in the facility. The Typhoon Trio has three excitation lasers (488/532/633) for direct excitation of red-, green-, and blue-excited fluorophores as well as chemifluorescent samples. With six emission filters, this allows for scanning of up to four fluorescent dyes in one sample
- Amersham Imager-600 RGB
This instrument is a multi-label imager capable of capturing and analyzing images from DNA gels, chemiluminescent and fluorescent Western blots (red, blue and green fluorophores), and Coomassie or silver-stained protein gels.
ImageQuant 5.2 and ImageQuantTL 8.1 software is available on computer in the facility for the analysis of 8 to 16 bit grayscale TIFF, .gel, or .ds files captured by these or other imagers.
Fees, Usage and Training
- Users pay an annual user fee, which allows for unlimited scanning on any of the instruments.
- Usage of the instruments must be recorded on the provided sign-in sheets.
- New users on any of the instruments must first attend a training session
For training on use of the imagers, please contact Dr. Linda Metheny-Barlow 336-713-7636.
Recent manuscripts including data acquired on Analytical Imaging Facility instrumentation:
- Manuse, MJ, and Parks, GD. TLR3-dependent upregulation of RIG-I leads to enhanced cytokine production from cells infected with the parainfluenza virus SV5. 2010. Virology 397: 231-241.
- Perrino FW, Harvey S, Shaban NM, Hollis T. RNaseH2 mutants that cause Aicardi-Goutieres syndrome are active nucleases. J Mol Med. 2009 Jan; 87(1):25-30.
- Gainey, MD, Dillon PJ, Clark KM, Manuse MJ, Parks GD. Paramyxovirus induced shut off of translation: role of P and V proteins in limiting activation of PKR. 2008. J. Virol 82:828-839.
- Creacy SD, Routh ED, Iwamoto F, Nagamine Y, Akman SA, Vaughn JP. G4 resolvase 1 binds both DNA and RNA tetramolecular quadruplex with high affinity and is the major source of tetramolecular quadruplex G4-DNA and G4-RNA resolving activity in HeLa cell lysates. J Biol Chem. 2008 Dec 12;283(50):34626-34.
- Lehtinen DA, Harvey S, Mulcahy MJ, Hollis T, Perrino FW. The TREX1 double-stranded DNA degradation activity is defective in dominant mutations associated with autoimmune disease. J Biol Chem. 2008 Nov 14;283(46):31649-56.
- Jiao Y, Wilkinson J 4th, Di X, Wang W, Hatcher H, Kock ND, D'Agostino R Jr, Knovich MA, Torti FM, Torti SV. Curcumin, a cancer chemopreventive and chemotherapeutic agent, is a biologically active iron chelator. Blood. 2009 Jan 8;113(2):462-9. Epub 2008 Sep 24.